Elisa Kits

ELISA kits are specialized tools designed for the detection and quantification of neurobiological markers. These kits utilize the Enzyme-Linked Immunosorbent Assay (ELISA) technique, which employs antibodies to specifically bind target antigens, facilitating their measurement.

Content:

Each AffiNEURO ELISA kit typically includes:

• Pre-coated 96-well microplate: Wells are pre-coated with capture antibodies specific to the target neurobiological marker.
• Detection antibodies: Biotinylated antibodies that bind to the target antigen, forming a sandwich complex.
• Enzyme conjugate: Streptavidin-HRP (horseradish peroxidase) that binds to the biotinylated detection antibody.
• Substrate solution: Typically TMB (3,3',5,5'-Tetramethylbenzidine), which reacts with HRP to produce a measurable color change.
• Stop solution: Usually an acidic solution to halt the enzymatic reaction.
• Standards and controls: Known concentrations of the target antigen for generating a standard curve and ensuring assay accuracy.

Application:

ELISA kits are employed in various research and clinical settings, including:

• Neuroscience research: Quantifying neurotrophic factors, neurotransmitters, or other neural proteins to study neural development, function, and disorders.
• Clinical diagnostics: Measuring biomarkers associated with neurological diseases such as Alzheimer's, Parkinson's, or multiple sclerosis.
• Pharmacological studies: Evaluating the effects of drugs on the expression levels of neural proteins.
• Biomarker discovery: Identifying and validating new biomarkers for neurological conditions.

These kits offer high sensitivity and specificity, enabling accurate quantification of neurobiological markers in various sample types, including serum, plasma, cerebrospinal fluid, and tissue extracts.

For detailed information on specific  ELISA kits, including target analytes and technical specifications, please refer to the manufacturer's website: