ELISA Kits AffiNEURO
AffiNEURO ELISA Kits are designed for the quantitative measurement of specific neurobiological targets in biological samples. These kits employ enzyme-linked immunosorbent assay (ELISA) technology to detect and quantify proteins associated with neurological functions or disorders.
Key Technical Details
Assay Principle
The AffiNEURO ELISA kits utilize a sandwich ELISA technique. Capture antibodies specific to the target neuroprotein are immobilized on a microplate. After sample incubation, the target proteins bind to these antibodies. A secondary, enzyme-conjugated antibody is then used to detect the bound target proteins. Substrate addition produces a colorimetric change proportional to the amount of target protein present.
Components
- Microplate: Pre-coated with capture antibodies specific to the neurobiological target.
- Standards: Known concentrations of the target protein used to generate a standard curve.
- Sample Diluent: Buffer used to prepare samples for accurate detection.
- Detection Reagent: Enzyme-conjugated secondary antibody that binds to the target protein.
- Substrate Solution: Chemical that reacts with the enzyme to produce a measurable color change.
- Stop Solution: Acidic solution that halts the color development reaction.
Procedure
- Sample Preparation: Dilute samples as needed.
- Incubation: Add samples and standards to the wells of the microplate and incubate to allow target proteins to bind.
- Washing: Remove unbound substances by washing the wells.
- Detection: Add enzyme-conjugated secondary antibody and incubate.
- Substrate Addition: Add substrate solution and incubate for color development.
- Stopping Reaction: Add stop solution to terminate the color development.
- Reading: Measure absorbance using a microplate reader at the specified wavelength.
Applications
These kits are used in research to study neurobiological markers, assess neurological disease states, and evaluate therapeutic interventions targeting neurobiological pathways.
Sensitivity and Specificity
The kits are optimized for high sensitivity and specificity for their respective targets, with validated performance characteristics.
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